绍兴黄酒麦曲中微生物的初步研究

【外文题名】Primary Study of Microorganism on Wheat Qu of Shaoxing Rice Wine

【作者】 方华

【关键词】 绍兴黄酒 麦曲 丝状真菌 ITS rDNA RISA

【外文关键词】 Shaoxing rice wine;Wheat Qu;Filamentous fungi;ITS rDNA;RISA

【导师姓名】陆健

【学位名称】硕士

【学位年度】暂无

【学位授予单位】暂无

【录入时间】2015-02-11

【全文挂接】 读秀挂接

【摘要】。分析了绍兴地区3个黄酒厂麦曲样品中真菌的RISA图谱,建立3个麦曲RISA图谱的克隆文库,通过条带的测序得到3个麦曲样品的主要微生物均有伞枝犁头霉、酿酒酵母、微小毛霉、米曲霉、构巢裸孢壳。同时,发现地域环境影响着麦曲中的优势微生物。采用RISA指纹图谱技术分析了在制曲过程中麦曲中数量占优势的真菌变化的过程。根据图谱条带的变化,发现在制曲过程中,麦曲中优势真菌先增加后下降。初步研究了麦曲7株优势真菌的产酶情况,其中Aspergillus oryzae AO-01产α-淀粉酶、糖化酶、蛋白酶,活力分别为112.5 u/g干曲、1359.4 u/g干曲、817.6 u/g干曲,能够用作为黄酒纯种酿造的菌株。而其它的6株真菌由于液化酶和糖化酶不产或产量很低,不能有效地将淀粉原料分解为酵母能利用的葡萄糖。在真菌混合...

【外文摘要】This paper studied on the microorganisms in the Wheat Qu of shaoxin and the character of the major filamentous fungus. Three mediums, PDA, MEA, CDA, were used to seperated mould from Wheat Qu. Sixteen kinds of mould were obtained. Chromosome DNA of every kind of the pure culture was extracted by benyl chloride lysis, then the ITS rDNA fragment was amplified and sequenced. The results of identification were obtained after blastn with the GenBank database. According to the quantity of the single clones, Absid...